FastQCFastQC Report
Sun 21 May 2023
EGAF00008004854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008004854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98436
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACT1298613.192328010077613No Hit
GCTCCTGGGAAGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC11711.189605428908123No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCCCTACTGAAGACGGGAGCGCT8990.9132837579747246No Hit
TGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATTGGCTCGATATATCATGAGTGATTCCAACT8600.8736641066276566No Hit
TGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACT6970.7080742817668333No Hit
AGCCTGAGGTTGCAGACTGGTCTGCAGCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTA6040.6135966516315169No Hit
GCTCCTGGGAACTCGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTAT4950.5028648055589419No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGACTGGA3070.3118777682961518No Hit
AGGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGCAGAGACTGCTGGGC2670.271242228453005No Hit
TGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACT2630.2671786744686903No Hit
AGAGACTTCCTCCACTGCCAAGCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCGGAAGCAATTCTTCACATA2310.23467024259417285No Hit
GCTCCTGGGAACTCGAGAACACCGAGATGAGGCCCGGGGCCGACGCGGCCATGGCGAGGGCGGCGGCGGGGAGGA1890.1920029257588687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA1420.14425616644317119No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG1410.14324027794709251No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG1310.13308139298630584No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC1230.12495428501767647No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCAGAAGAAATTCTTCACATAATTG1180.1198748425372831No Hit
AGCCTGAGGTTGCAGCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1150.11682717704904709No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG1140.11581128855296843No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACA1120.11377951156081108No Hit
GTATAAGAGACAGAGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTC1090.11073184607257507No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG1030.10463651509610306No Hit
GCTCCTGGGAACTCGAGAACACCGAGATGAGGCCCGGGGCCGGCGCGGCCATGGCGAGGGCGGCGGCGGGGAGGA1010.10260473810394571No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)