FastQCFastQC Report
Sun 21 May 2023
EGAF00008004918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008004918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25586
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCTGGGAAGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1890.7386852184788556No Hit
GTCTGCAGCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG1430.5588993981083406TruSeq Adapter, Index 9 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG940.3673884155397483TruSeq Adapter, Index 9 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG880.34393809114359414TruSeq Adapter, Index 9 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG620.24232001876025952TruSeq Adapter, Index 9 (96% over 30bp)
GCTCCTGGGAACTCGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTAT590.23059485656218245No Hit
AGCCTGAGGTTGCAGCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG500.19541936996795123No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGTAGTTCCCTACTGAAGACA480.1876025951692332No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACT470.18369420776987416No Hit
AGAGACTTCCTCCACTGCCAGAAATCTTGAATAGCCTCCTGCTCAGTCATCCAGAAGCAATTCTTCACATAACTG450.1758774329711561No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGGTGCAGGTATCCTCTGTGCCTATTCAGCAGTTCCCTACTGAAGACTG330.1289767841788478No Hit
GTGTATAAGAGACAGGGTGGTTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC320.12506839677948878TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTGGGG310.12116000938012976TruSeq Adapter, Index 9 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG310.12116000938012976TruSeq Adapter, Index 9 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG290.11334323458141171RNA PCR Primer, Index 9 (96% over 30bp)
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTGCAGGTGCCCTCTGTGCCTATTCAGCAGTTCCCTACTGAAGACT290.11334323458141171No Hit
GAGATGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTG280.10943484718205268No Hit
ACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG280.10943484718205268RNA PCR Primer, Index 9 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG280.10943484718205268TruSeq Adapter, Index 9 (96% over 30bp)
GTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTC280.10943484718205268No Hit
GAGATGTGTATAAGAGACAGGGTGGTTCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATG260.10161807238333463No Hit
ACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGG260.10161807238333463RNA PCR Primer, Index 9 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)