FastQCFastQC Report
Tue 30 May 2023
EGAF00008005168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGG80.46029919447640966No Hit
TGTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGG70.4027617951668585No Hit
GAGATGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA50.28768699654775604No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGT50.28768699654775604No Hit
GTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGG50.28768699654775604No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTA40.23014959723820483No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG30.17261219792865362No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGGGTT30.17261219792865362No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATT30.17261219792865362No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCGCGGT30.17261219792865362No Hit
GTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGTTG30.17261219792865362No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC30.17261219792865362No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC30.17261219792865362No Hit
GTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGC30.17261219792865362No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGT30.17261219792865362No Hit
TGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCG30.17261219792865362No Hit
TGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGG30.17261219792865362No Hit
CGGAGATGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC30.17261219792865362No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGA30.17261219792865362No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGCGGT30.17261219792865362No Hit
GAGATGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGG20.11507479861910241No Hit
GTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGGGG20.11507479861910241No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAG20.11507479861910241No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTG20.11507479861910241No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTGTTGTGCTTGAAAAAAAAAATGGGG20.11507479861910241No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGG20.11507479861910241No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG20.11507479861910241No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCGGGGT20.11507479861910241No Hit
CTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATG20.11507479861910241No Hit
CTTCCAAGCCAGGTTATTTTTGGAGGAAAACTGACAGCAATGGAGAACATTCCCGAACTCTTTCTTAGAGGGCTT20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAGTCGGGGG20.11507479861910241No Hit
GGGTACTTGGGCACAGGCCTCTCCCCACCCACGGCCTGCACGTAGCTCTCGAAGCTTGGGGGCAGCCCCAGATGC20.11507479861910241No Hit
GGAGATGTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG20.11507479861910241No Hit
CCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA20.11507479861910241No Hit
TGTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGGGG20.11507479861910241No Hit
GAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAA20.11507479861910241No Hit
CCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAAC20.11507479861910241No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACG20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTT20.11507479861910241No Hit
GTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGGGTTTT20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGGT20.11507479861910241No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT20.11507479861910241No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGT20.11507479861910241No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA20.11507479861910241No Hit
GTATAAGAGACAGCGTAGGGAGCGCTTCCTACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA20.11507479861910241No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGG20.11507479861910241No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGT20.11507479861910241No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTT20.11507479861910241No Hit
GTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATG20.11507479861910241No Hit
GTATAAGAGACAGCGTAGGGAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATAGCGTAT20.11507479861910241No Hit
TGTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGGGGGG20.11507479861910241No Hit
CCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTC20.11507479861910241No Hit
GTGTATAAGAGACAGGGGAGCTGCAGACCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTG20.11507479861910241No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTG20.11507479861910241No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCA20.11507479861910241No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGGGGG20.11507479861910241No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGC20.11507479861910241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)