FastQCFastQC Report
Tue 30 May 2023
EGAF00008005242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31039
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACA15254.91317374915429No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG7872.535519829891427No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA7612.4517542446599436No Hit
GATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG5621.8106253423112857No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA3000.9665259834401881No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA1290.4156061728792809No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC940.3028448081445923No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG930.29962305486645835No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC900.28995779503205643No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG870.28029253519765457No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAGG820.2641837688069848No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAG810.26096201552885084No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT700.22552272946937724No Hit
GTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAG600.1933051966880376No Hit
AGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCA570.18363993685363575No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGCGCCCACGAGACA540.17397467701923386No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACC450.14497889751602822No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC450.14497889751602822No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGCCA450.14497889751602822No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCT420.13531363768162633No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACGA410.1320918844034924No Hit
GCGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC400.12887013112535842No Hit
ATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA380.12242662456909051No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC370.11920487129095654No Hit
TGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA320.10309610490028674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)