FastQCFastQC Report
Sun 21 May 2023
EGAF00008005248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23986
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACG1090.4544317518552489No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1020.425248061369132RNA PCR Primer, Index 31 (95% over 21bp)
GATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACG410.17093304427582756No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGGCT400.16676394563495373No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTA360.15008755107145835No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTGT350.14591845243058452No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATTCCGTCT340.14174935378971065No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATC270.11256566330359376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)