FastQCFastQC Report
Tue 30 May 2023
EGAF00008005250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11502
Sequences flagged as poor quality0
Sequence length75
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4223.668927143105547RNA PCR Primer, Index 33 (95% over 21bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCG790.6868370718135977No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTGT780.6781429316640584No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACA590.5129542688228134No Hit
GGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT590.5129542688228134RNA PCR Primer, Index 33 (95% over 21bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGGCT540.4694835680751174No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATTCCGTCT490.42601286732742133No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA420.3651538862806468No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGTCT420.3651538862806468No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTTTGCCGTCT390.3390714658320292No Hit
GATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG330.28690662493479396No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCT320.27821248478525473No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGTCT300.2608242044861763No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGGCG270.2347417840375587No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTGG250.21735350373848025No Hit
AGAGACTTCCTGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT250.21735350373848025RNA PCR Primer, Index 33 (95% over 21bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGTGT220.19127108328986264No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGTCG200.1738828029907842No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGTCG200.1738828029907842No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA190.165188662841245No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCA170.14780038254216657No Hit
TGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT170.14780038254216657RNA PCR Primer, Index 33 (95% over 21bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGGGT170.14780038254216657No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATGTCGTATGCCGTCT170.14780038254216657No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC170.14780038254216657No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATG160.13910624239262737No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTTTGCCGTGT150.13041210224308816No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCCTCT130.11302382194400974No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATTCCGGCT130.11302382194400974No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATTCCGTGT130.11302382194400974No Hit
GGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCG130.11302382194400974No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGGCT120.10432968179447052No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGGATGCCGTCT120.10432968179447052No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTTTGGCGTCT120.10432968179447052No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGTGG120.10432968179447052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)