FastQCFastQC Report
Tue 30 May 2023
EGAF00008005257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24985
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGTCT1570.6283770262157294No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACG1080.43225935561336803No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCGGAAGCAATTCTTCACGTAATTG550.22013207924754855No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGTGT540.21612967780668402No Hit
GATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGG460.18411046627976785No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGTCG400.16009605763458074No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGG390.15609365619371623No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTA390.15609365619371623No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT370.14808885331198718No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCCGTCT340.13608164898939362No Hit
GGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTC340.13608164898939362No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA330.13207924754852912No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGGCT320.1280768461076646No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGGCGTCT310.12407444466680008No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATG300.12007204322593557No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTTTGCCGTCT290.11606964178507104No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTA280.11206724034420652No Hit
GATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTC270.10806483890334201No Hit
AGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTT270.10806483890334201No Hit
GGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGTCT260.1040624374624775No Hit
GTATCAACGCAGAGTACATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCG250.10006003602161297No Hit
GTACATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGA250.10006003602161297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)