FastQCFastQC Report
Tue 30 May 2023
EGAF00008005258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25657
Sequences flagged as poor quality0
Sequence length75
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT1820.7093580699224383No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACA1760.6859726390458745No Hit
GATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTG750.2923178859570488No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTGT610.23775188057839966No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATG510.19877616245079316No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGA480.1870834470125112No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCG470.18318587519975055No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCGCGTATGCCGTCT460.1792883033869899No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATGTCGTATGCCGTCT430.16759558794870796No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGGCGTCT400.155902872510426No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGGCGTGT380.1481077288849047No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGGCT380.1481077288849047No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGA310.12082472619558016No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGT300.1169271543828195No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG290.11302958257005885Clontech Universal Primer Mix Long (96% over 25bp)
AGAGACTTCCTCCACTGCCAGGATCTTGAATAGTCTCCTGGTCAGTTGTCCGGAAGCAATTCTTCACATAATTGA270.10523443894453754No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTTTGCCGTCT270.10523443894453754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)