FastQCFastQC Report
Sun 21 May 2023
EGAF00008005312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724072
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGG43320.5982830436752147No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCG30730.42440530775944935No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGG22960.31709553746036306No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG21770.3006607077749174No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGG19960.2756631937155421No Hit
AGAGACTTCCTCCACGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGG14100.1947320156006585No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTG12550.17332530466583435No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG11990.1655912671668011No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG11850.16365775779204278No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG10960.15136616248107923No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG10750.14846589841894178No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC10310.14238915466970137No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10270.1418367234197704No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGT10190.14073186091990852No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA10170.14045564529494306No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG9260.12788783435901402No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG9110.125816217171773No Hit
CTGATGAGGCCCGTGTACTCTGCGTTGATACCACTGCTTCTGCTCTTCGGCCTCCTGTCTCTTATACACATCTCC9100.12567810935929022Clontech SMART CDS Primer II A (96% over 26bp)
AGAGACTTCCTCCACGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCG9050.12498757029687656No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTT9050.12498757029687656No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA9020.12457324685942835No Hit
AGAGACTTCCTCCACTTTGGGAAGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA8510.11752974842280878No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGT8460.1168392093603951No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGG7940.10965760311129279No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG7910.1092432796738446No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT7870.10869084842391363No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGGG7770.10730977029908628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)