FastQCFastQC Report
Sun 21 May 2023
EGAF00008005336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774261
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCTCCACTGCTCTTCGGCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCG67210.8680535375022117No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGG54920.7093215336947102No Hit
AGAGACTTCCTCCACTGCTCTTCGGCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT28720.370934349011509No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTG28000.36163515920342104No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGG19760.2552110980664143No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGG16860.21775602800605995No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGC15500.2001908917018938No Hit
AGAGACTTCCTCCACTGCTCTTCGGCCCCCGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA13050.16854781527159446No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG12570.1623483553995358No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG12540.16196088915753215No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG11840.15292001017744664No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCG11750.15175761145143563No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC11210.14478321909536965No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA10870.14039193501932812No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGT10530.13600065094328656No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTG10350.1336758534912646No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG10300.13303007642125847No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC10120.13070527896923645No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG10030.12954288024322547No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG9330.12050200126313994No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9310.12024369043513751No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTT9300.12011453502113628No Hit
AGAGACTTCCTCCACTGCTCTTCGGCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATAG9280.11985622419313385No Hit
AGAGACTTCCTCCACTGCTCTTCGGCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGG9000.11623987260109964No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG8990.1161107171870984No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA8530.1101695681430422No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGT8270.10681152737901044No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG8050.10397010827098356No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT8000.10332433120097745No Hit
AGAGACTTCCTCCACTGCCTAGTCCCGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGG7800.10074122292095301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)