FastQCFastQC Report
Tue 30 May 2023
EGAF00008005605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008005605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7859
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT2423.079272172032065No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACT2192.786614073037282No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGA831.0561140094159562No Hit
GATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA760.9670441532001526No Hit
GACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC630.8016287059422318No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATG560.7125588497264284No Hit
CTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC460.5853161979895661TruSeq Adapter, Index 1 (96% over 25bp)
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC440.5598676676421938No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATC350.4453492810790177No Hit
CAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG320.407176485557959No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCGTCT280.3562794248632142No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGA270.34355515968952793No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG250.3181066293421555TruSeq Adapter, Index 1 (96% over 28bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA240.30538236416846926No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGG220.2799338338210969No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCG200.2544853034737244No Hit
GAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT200.2544853034737244No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGC190.24176103830003814No Hit
GTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGT190.24176103830003814No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGG190.24176103830003814No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG180.22903677312635196TruSeq Adapter, Index 1 (96% over 28bp)
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA160.2035882427789795TruSeq Adapter, Index 1 (96% over 28bp)
AGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG150.1908639776052933No Hit
GACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT150.1908639776052933TruSeq Adapter, Index 1 (95% over 23bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCT140.1781397124316071No Hit
GATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAA140.1781397124316071TruSeq Adapter, Index 1 (96% over 28bp)
AGAGACTTCCTCCACTGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC140.1781397124316071No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAG130.16541544725792084No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGC130.16541544725792084No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG120.15269118208423463TruSeq Adapter, Index 1 (96% over 28bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGTCT120.15269118208423463No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG120.15269118208423463No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCCCGAGACT120.15269118208423463No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG120.15269118208423463TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGG110.13996691691054844RNA PCR Primer, Index 1 (96% over 28bp)
CTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGG110.13996691691054844TruSeq Adapter, Index 1 (95% over 24bp)
AGAGACTTCCGGATCAACTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA100.1272426517368622No Hit
GACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGC100.1272426517368622No Hit
AGAGACTTCCGGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT100.1272426517368622TruSeq Adapter, Index 1 (95% over 23bp)
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTC90.11451838656317598No Hit
AGAGACTTCCTCCACGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCG90.11451838656317598No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG90.11451838656317598RNA PCR Primer, Index 1 (96% over 28bp)
AGAGACTTCCGGATCAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG90.11451838656317598No Hit
GTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATA90.11451838656317598No Hit
AGAGACTTCCTCCACTGCCTAGTCCCGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC80.10179412138948975No Hit
AGAGACTTCCTCCACGCCTAGTCCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCG80.10179412138948975No Hit
ACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC80.10179412138948975No Hit
AGAGACTTCCTCCACTGCCTAGTCCCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTC80.10179412138948975No Hit
CGTCAGATGTGTATAAGAGACAGAGAGCCTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACT80.10179412138948975No Hit
ATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC80.10179412138948975No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCTGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACT80.10179412138948975No Hit
AGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGCTCTCGTATGCCGTCT80.10179412138948975No Hit
CGTCAGATGTGTATAAGAGACAGAGAGACTTCCGGATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGCCT80.10179412138948975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)