FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008008937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008008937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108094382
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC2377230.21992169768822956No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCTCGTATG1806090.16708453913913862TruSeq Adapter, Index 21 (97% over 38bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1358700.12569570914425504No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1302620.1205076504345989No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT1275740.11802093470500623No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1114260.10308213797827162No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1109020.10259737642979447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)