FastQCFastQC Report
Wed 8 May 2024
EGAF00008010125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008010125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670516
Total Bases277.8 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC96240.16972000431706744Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC92780.1636182668385029Illumina Single End PCR Primer 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)