FastQCFastQC Report
Wed 31 May 2023
EGAF00008011167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26463971
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5312992.0076314321837794No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT4375781.6534857901710969No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3432721.2971295955546505No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT3232761.221570262452298No Hit
TGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA1127630.42610007394581867No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT824140.3114196278404326No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT822050.3106298748589167No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA754950.2852746475576171No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT724190.27365129745645506No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA697760.2636641341543187No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG644240.24344041187167265No Hit
TGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGG616430.2329317848783918No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT585230.22114217099164749No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469620.1774563613299002No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464000.17533271934132635No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA462390.17472434503499115No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461120.17424444729024227No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446850.16885221042601656No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT439140.16593881545592684No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA399470.15094862369672335No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT392260.14822416484661355No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG375430.14186457504809086No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366810.13860731633963777No Hit
CGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCA356230.13460942804086354No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA352880.13334355603699838No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352260.13310927524822333No Hit
GTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGA350020.13226284143071348No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC344950.13034702917411753No Hit
TTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324420.12258931208774375No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323440.12221899729258319No Hit
GAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATA322440.12184112505262343No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT312000.11789613886744359No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC309320.11688344126435145No Hit
TGTGTTGTGATAAGGGTGGAGAGGTTAAAGGAGCCACTTATTAGTAATGT306790.11592742449725325No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG303660.11474468438617924No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA300210.11344102515831807No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG278450.1052185252167938No Hit
TGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGA271580.10262254292827029No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC270910.10236936852749726No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA267980.10126220286441516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)