FastQCFastQC Report
Wed 31 May 2023
EGAF00008011173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22492937
Sequences flagged as poor quality0
Sequence length35-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT5784312.571611701931144No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT3720511.6540792338501638No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1985220.8825970570228334No Hit
TGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA1414920.6290507993687084No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT1130540.5026200002249596No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT909530.4043624894339054No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA904890.40229961965393846No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT833680.37064079270750633No Hit
TGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGG703470.3127515095071844No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA679570.30212595180433754No Hit
GCCGCCCACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586210.2606195891625891No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA509490.22651110435244629No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT500740.2226209943147931No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT493910.21958448556540214No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA493190.21926438508230386No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG464850.20666487440035067No Hit
GAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATA444430.19758646903247895No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA427860.19021971208117464No Hit
CGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCA414130.18411557370209147No Hit
TGCCGCCCACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371170.16501624487722524No Hit
TGTGTTGTGATAAGGGTGGAGAGGTTAAAGGAGCCACTTATTAGTAATGT361640.1607793593162156No Hit
GTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGA340320.15130082834447098No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT333350.14820207783447756No Hit
TGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGA328300.1459569286127463No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC323660.14389405883277936No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG318510.1416044512106178No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG279840.12441238776421239No Hit
TGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC279060.12406561224085587No Hit
TGTTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA278080.12362991991663871No Hit
TGGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTG273720.12169153365787669No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA259540.11538733247685706No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC258730.11502721943337146No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC255850.1137468175009782No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA250830.11151500579937605No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA249470.11091037155352367No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC247950.11023460386698276No Hit
GGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTA238110.10585989726463911No Hit
GGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCA236400.10509965861728063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)