FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008011221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25081701
Sequences flagged as poor quality0
Sequence length35-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT6348722.5312158852384052No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT4190191.6706163589144132No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT2081980.8300792677498229No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1890310.7536610056869748No Hit
TGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA1580050.6299612613992966No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA1520050.6060394388721881No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT1070700.42688492299625136No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA949250.3784631672309625No Hit
TGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGG795090.3170000312179784No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647940.2583317614702448No Hit
TGTGTTGTGATAAGGGTGGAGAGGTTAAAGGAGCCACTTATTAGTAATGT540170.21536418124113674No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA527870.2104602076230795No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA526170.20978242265147806No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT515970.2057157128218696No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT509340.20307235143262412No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA495650.19761418892602223No Hit
GTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGA493610.1968008469601005No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA461720.18408639828694234No Hit
TCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGG447900.17857640516486503No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG430550.17165901148410947No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT427030.17025559789585243No Hit
CCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTT399850.1594190122910723No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC396870.15823089510555924No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT368020.1467284854404412No Hit
CGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCA358540.14294883748115805No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG356490.1421315085448152No Hit
TGGGCAGTGAGGGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTG346990.1383438866446897No Hit
TGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGA344360.1372953134239181No Hit
GTGGGTAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT316090.12602414804322878No Hit
TGTTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA312410.12455694292823281No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT310690.12387118401578905No Hit
GAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATA303090.12084108649568863No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT301820.12033474125219816No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC298800.11913067618500037No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA291160.11608463078321522No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC290870.11596900864100086No Hit
TGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC285730.1139197058445119No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA283730.11312231176027496No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG283170.11289904141668859No Hit
ATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCG268500.10705015580881058No Hit
TGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTT265160.10571850768813487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)