FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008011237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25153307
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT4418421.7565960611063987No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT3278741.303502557337689No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2394240.9518589344931861No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT1556990.6190001179566568No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA1324700.5266504320883135No Hit
TGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA1167750.4642530701827795No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1087730.43244015588089474No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT882650.3509081330737147No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG737860.293345125553471No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC700180.27836498795168363No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA696810.277025203882734No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA602790.2396464210451532No Hit
TGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGG594390.23630689992373569No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA505450.20094773224053603No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG503130.20002538831176356No Hit
GTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGA439670.17479610136353044No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC419970.16696412920972978No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT409440.16277780094680988No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA406630.16166065161928808No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT400990.15941840172347915No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT391040.1554626594427524No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA380490.15126837993906725No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT373120.1483383477170616No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG360650.1433807490999096No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT340970.1355567281868742No Hit
GAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATA334910.13314750223499439No Hit
CGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCA325390.12936271163072116No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT322240.12811039121018958No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG314000.12483448001489428No Hit
TGGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTG303870.12080717656727999No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA302570.1202903459175368No Hit
TGTGTTGTGATAAGGGTGGAGAGGTTAAAGGAGCCACTTATTAGTAATGT298820.11879948827404684No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA284710.11318988791414186No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC281320.11184215260442693No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG269910.10730596974783475No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC264360.10509950043546958No Hit
TGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGA256260.10187924792553123No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT255110.10142205158152763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)