FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008011241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19601654
Sequences flagged as poor quality0
Sequence length35-101
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG3873531.9761240556536708No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA2296931.171804175300717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1702040.8683144799923517No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1581550.8068451774528822No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1451120.7403048742723446No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1243680.6344770701492843No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1163810.5937305086601365No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1159620.5915929339432274No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1010860.5157013790775002No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC804800.4105775971762383No Hit
GATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCA804620.41048576819078636No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT756080.3857225517805793No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG715930.3652395864144934No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG679490.346649318470778No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA670580.3421037836909069No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT647740.33045170575911603No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA631800.32231973893631627No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGA605970.3091422795239626No Hit
TGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG503120.25667221755878356No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT469630.2395869246544195No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA410720.20953333836012003No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT407960.20812529391652357No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG388570.19823327153922826No Hit
GCTTCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG365130.18627509698926428No Hit
GCCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT350520.17882164433674833No Hit
GGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG346470.17675549216407963No Hit
GCTCCATTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG341860.17440364981444934No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA340170.17354147767326167No Hit
GGTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG339450.1731741617314539No Hit
CCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCT335300.17105699345575634No Hit
GCACCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG325640.1661288379031688No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG322080.16431266463534147No Hit
AAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATC321260.16389433259050484No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC309080.15768057124159013No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCC304480.1553338305022627No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA301340.15373192486715662No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGC287770.14680903968614076No Hit
CCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGC281390.14355421231289972No Hit
GCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT279430.14255429669353412No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA275710.14065649766086066No Hit
TCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGC267660.1365497013670377No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCT267440.1364374659403742No Hit
ACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGT259080.1321725197271618No Hit
GATCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG255430.13031043196660852No Hit
GGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG254810.12999413212782962No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC246300.12565266176007392No Hit
GGGTCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA243180.12406095934557358No Hit
CCCATCCAAGTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTA241330.12311716143953975No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG238160.12149995097352499No Hit
GCTACGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG236700.12075511586930368No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG231500.11810227851180315No Hit
CCTGGGCTCAAGCGATCCTCCCACCTCAGCCTCCCGAGTAGCTGGGACTA229130.11689319687001923No Hit
GCCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG224910.11474031732220148No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG223360.11394956772525422No Hit
CCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA217240.11082738221988818No Hit
ACTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG211980.10814393520057032No Hit
GCTGCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG210930.10760826611876734No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA209650.10695525999999797No Hit
GGTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA208990.1066185537200075No Hit
GTGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGG205860.10502174969520428No Hit
TCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA205620.10489931104793503No Hit
CCTGGACTCAAGCGATCCTCCAGCCTCAGCCTCCCGAGTAGCTGGGACTA205040.10460341765036767No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT204970.10456770637824746No Hit
TCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG203220.10367492457524247No Hit
GCTCCTTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG202730.10342494567040109No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG200860.10247094454376147No Hit
CGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG196650.10032316660624659No Hit
GCCCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG196270.10012930541473694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)