FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008011259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27230426
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4672071.7157535471534673No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2485250.9126739331951692No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1892050.6948293794595795No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA1542130.5663260648217548No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT1511660.555136375758499No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT1085140.39850276304895116No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1075360.39491119235519856No Hit
CTCTGCAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA940340.34532695155044585No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA733300.26929435477799724No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC683450.25098762685534187No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGA683080.2508517494364576No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG630240.23144698507471018No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTGTCCA626730.23015798577664556No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG625050.22954102884765742No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT586690.21545384563576053No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA583660.21434111974597825No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT567900.20855347617404152No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC567550.2084249434805023No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA550620.2022076334758773No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT545720.20040817576632847No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT532580.19558269121459945No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG528300.1940109199907486No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA508260.18665150519496096No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA458010.16819788276540368No Hit
GCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGC449300.16499925487761374No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTGGGTGC413130.15171631909100503No Hit
TGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG407850.14977731159989932No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC403230.14808068004518182No Hit
CTGCAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC402190.14769875432723675No Hit
GGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCC399020.1465346153600388No Hit
AAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATC390880.14354531214458413No Hit
CTCTCTGCAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC388740.14275942653265872No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC377110.13848846874448456No Hit
CCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCT370010.13588109124697498No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG343670.12620808796748167No Hit
GGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGT340550.12506231081364647No Hit
GATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCA324370.11912042800946265No Hit
TGCCGAGTTCCTTTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322660.11849245399245681No Hit
CCTAAGTGTAAGTTGGGTGCTTTGTGTTAAGCAACACTCTGGTCAGAGCG307640.11297656525828864No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT303740.11154434381599465No Hit
GGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTG281630.10342474994698946No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA279760.10273801812722284No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC273340.10038036129144656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)