FastQCFastQC Report
Wed 31 May 2023
EGAF00008011261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34015154
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15491164.554193698490973No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4215761.239377014139051No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT1901690.5590714068206188No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT1754090.515678982373562No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1703840.5009061549449402No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1703010.5006621460540793No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1586770.4664891418689447No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1493270.43900139332016547No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA1254440.3687885699415031No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160280.34110679022649726No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT1152270.33875195743638264No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA1056550.3106115586012046No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA990780.2912760589001008No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA911690.26802465748060406No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT836210.24583454774304417No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT834320.24527891303975868No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC828620.24360318933143738No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG816190.23994893570083498No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT751550.2209456408752405No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA724100.2128757082799037No Hit
GCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGC684420.20121031937706352No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT630360.18531740294340576No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT626930.18430902885225803No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA612800.18015499797531417No Hit
CCTAAGTGTAAGTTGGGTGCTTTGTGTTAAGCAACACTCTGGTCAGAGCG611130.17966404032743757No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC534410.15710938718666392No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG524660.154243017685588No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG517710.152199810707898No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC508060.14936283986837162No Hit
GGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGT502780.1478105905385582No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479110.1408519273497924No Hit
TGCCGAGTTCCTTTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476070.1399582080386877No Hit
GGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCC471830.13871170478898903No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG460980.1355219500108687No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA458260.1347223064167224No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT434900.12785477907876003No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433060.1273138437062493No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC433000.12729620450931958No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT397300.11680088233614935No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393780.11576604944960708No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC393440.1156660940003388No Hit
TGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGA384830.11313486924092715No Hit
TCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGG383180.11264979132536046No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA374130.10998921245513103No Hit
GGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCA370490.10891910117472936No Hit
TTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364750.10723161800178825No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT363850.10696703004784279No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTGGGTGC354760.10429469171299358No Hit
GGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTA353300.10386547125437091No Hit
CGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCA349400.10271892345394057No Hit
TGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA341740.10046698597924912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)