FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008011265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008011265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16905490
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT3730412.206626368120652No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2414851.428441293331338No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1246710.7374586598791281No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1222900.7233744777584087No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT975240.5768776888454579No Hit
TGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA941930.557174030448097No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT847890.5015471305475322No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA719000.425305625568972No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA619710.3665732256207894No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA513210.30357593894054535No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG510310.3018605198666232No Hit
TGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGG491400.2906748044570137No Hit
GGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAA468870.2773477728240944No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463060.27391101943806423No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA432830.2560292544019724No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC383520.22686121490711006No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG377680.22340671580652205No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTGGGTGC352040.20824004509777594No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC332960.19695377063900543No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG315850.1868327981028648No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG314480.18602241047139123No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC309970.18335463804953303No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT291980.1727131245530298No Hit
CCTAAGTGTAAGTTGGGTGCTTTGTGTTAAGCAACACTCTGGTCAGAGCG289600.17130529786477647No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA278420.16469206157289734No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT277560.1641833510889066No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT269560.15945116054015587No Hit
GCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGC268730.158960195770723No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA257740.1524593490043767No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGA240570.14230288503912042No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC234500.13871233546025583No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA225720.13351875633300186No Hit
GGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGT224420.13274977536882987No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT222030.13133603344239062No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT221130.13080366200565616No Hit
GGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCC220040.13015890104338887No Hit
GGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTA218210.12907641245536214No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC214320.1267753848010321No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG208910.12357524094243941No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT204220.12080099423323429No Hit
TGTTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAA202590.11983681040892633No Hit
TCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGG182070.10769874165138071No Hit
TGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC176090.10416142921618954No Hit
TGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG174200.10304344919904718No Hit
GATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCA172590.10209109585111109No Hit
TGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGA172560.10207335013655326No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA172100.10180124918000011No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG170620.10092579392848122No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG170450.10082523487932027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)