FastQCFastQC Report
Wed 31 May 2023
EGAF00008012461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008012461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55013
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA25904.707978114263901No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19653.571883009470489No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10691.9431770672386526No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9481.723229054950648No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8501.5450893425190408No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8241.497827786159635No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8141.4796502644829406No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7891.434206460291204No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7471.3578608692490866No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7411.3469543562430697No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7361.3378655954047225No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7331.3324123389017142No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7231.3142348172250196No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7091.2887862868776472No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6931.259702252194936No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6911.2560667478595968No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6191.1251885917873956No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6051.0997400614400232No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5901.0724737789249812No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5701.0361187355715922No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5671.0306654790685839No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5611.019758966062567No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5040.9161470925054078No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4980.9052405794993911No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4720.8579790231399851No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4660.8470725101339683No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4640.8434370057986295No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4480.814352971115918No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4000.727100867067784No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3990.7252831149001145No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3960.7198298583971061No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3850.6998345845527421No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3700.6725683020377002No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3660.6652972933670224No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3560.6471197716903277No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3430.6234889935106248No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.6180357370076164No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.6071292240015996No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3320.6034937196662606No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3280.5962227109955829No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3250.5907694544925745No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3240.588951702324905No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3160.5744096849835494No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3160.5744096849835494No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2940.5344191372948213No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2910.5289658807918128No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2910.5289658807918128No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2750.49988184610910147No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.4944285896060931No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2700.4907930852707542No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2670.48533982876774584No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2640.47988657226473747No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2630.4780688200970679No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2620.4762510679293985No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2490.45262028974969554No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2480.4508025375820261No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.4253540072346536No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2340.4253540072346536No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2200.3999054768872812No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2190.39808772471961174No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2160.39263446821660336No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2130.387181211713595No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2060.37445694653990874No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2030.36900369003690037No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2020.3671859378692309No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2000.363550433533892No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1860.33810190318651956No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.3253776380128333No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.31447112500681657No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.31447112500681657No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1660.3017468598331303No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1640.29811135549779144No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1620.2944758511624525No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1610.29265809899478307No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1580.2872048424917747No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1560.28356933815643576No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1560.28356933815643576No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1520.2762983294857579No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.2708450729827495No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2690273208150801No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.2690273208150801No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1460.2653918164797412No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.2635740643120717No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1370.24903204697071601No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1370.24903204697071601No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1370.24903204697071601No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1360.24721429480304655No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1340.24357879046770764No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1340.24357879046770764No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1330.24176103830003814No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1330.24176103830003814No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1320.23994328613236873No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1310.23812553396469924No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1300.23630778179702977No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1300.23630778179702977No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1280.23267227746169086No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1220.22176576445567409No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.21994801228800465No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.21631250795266574No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.21631250795266574No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.21449475578499627No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1180.21449475578499627No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.205405994946649No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1120.2035882427789795No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.1944994819406322No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1070.1944994819406322No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1060.19268172977296275No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATGCGTAGGGTGCGAGCGTTA1030.18722847326995437No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1010.18359296893461546No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.181775216766946No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT990.17995746459927653No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.17632196026393762No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA960.17450420809626815No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC960.17450420809626815No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT920.1672331994255903No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT920.1672331994255903No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC910.16541544725792084No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT900.1635976950902514No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA890.16177994292258194No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC880.15996219075491247No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG880.15996219075491247No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.158144438587243No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC860.15632668641957356No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC840.15269118208423463No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.15087342991656516No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA830.15087342991656516No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT820.14905567774889572No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT820.14905567774889572No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC810.14723792558122625No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC810.14723792558122625No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA810.14723792558122625No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.14178466907821788No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA770.13996691691054844No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT750.1363314125752095No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATGCGTAGGGTGCGAGCGTTA750.1363314125752095No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.13451366040754004No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG730.1326959082398706No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.1272426517368622No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA690.12542489956919275No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC680.12360714740152327No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.11633613873084543No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC640.11633613873084543No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC640.11633613873084543No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA620.11270063439550652No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC620.11270063439550652No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA620.11270063439550652No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC610.11088288222783704No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.11088288222783704No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA610.11088288222783704No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG610.11088288222783704No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.10906513006016759No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.10906513006016759No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA600.10906513006016759No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.10906513006016759No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC590.10724737789249814No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.10724737789249814No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.10724737789249814No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.10542962572482868No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT570.1036118735571592No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.1036118735571592No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC560.10179412138948975No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT560.10179412138948975No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA560.10179412138948975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)