FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008014711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008014711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9231108
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1072601.1619406901100062No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1025521.110939228530313No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT982251.0640651154769287No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC885750.9595272853486276No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA717160.7768948212933918No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC703290.7618695393879045No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT566550.6137399757428903No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC520480.5638326406754206No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG508390.5507356213360303No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT474330.5138386421218342No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC403880.43752060966029216No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA386060.41821631812779136No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC386020.418172986384733No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT356580.3862808234937778No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG353760.3832259356081632No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA338480.36667320975986845No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG338120.3662832240723432No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG333720.3615167323359233No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT324850.3519079183127312No Hit
CCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCC317350.34378321648928817No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT316080.3424074336471851No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC308020.33367608742092497No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC279100.3023472371897285No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC277420.3005273039812772No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT276900.2999639913215185No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT274920.29781907004012953No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG266730.28894689564892967No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC266580.28878440161246083No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA264410.2864336545515446No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG248270.2689492962274951No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT241780.2619187209162757No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA240010.2600012912859431No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT236270.2559497733099862No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG224040.24270109286989167No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA217240.23533469654996994No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC213490.2312723456382484No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG204830.2218910232661128No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGC199560.21618206611817345No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC195230.21149140493210566No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG193380.20948731181565636No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG192730.20878317099095794No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC192520.20855567933990155No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA192290.208306521817316No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG191820.2077973738363802No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC188550.20425500384135903No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT187230.20282505632043304No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT186020.20151427109291756No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC184980.20038764577340012No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT178120.19295625183889084No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA178110.19294541890312625No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC164810.17853761433622053No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC164530.17823429213481198No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA164270.17795263580493265No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC163370.17697767158611946No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG158360.17155037076805948No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCC157870.17101955691559453No Hit
CCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCC152600.1653105997676552No Hit
CCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA140940.15267939666614236No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC140780.1525060696939089No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG138770.15032864960522616No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT137790.14926702190029625No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC134720.1459413106205669No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG128790.13951737971216455No Hit
GCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACT126760.13731829375195265No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC124320.13467505742539249No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC124090.1344258999028069No Hit
GCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACT121690.1318259953193051No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG121670.13180432944777593No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT119300.12923692367156792No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT117650.12744948927041044No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA117260.1270270047755914No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA117140.1268970095464163No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC113280.12271549634128429No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG111020.12026725285848676No Hit
CGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGC110980.1202239211154284No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT109050.11813316451286238No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC107340.11628073249711736No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC107030.11594491148841504No Hit
GGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCG106750.1156415892870065No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC106460.11532743414983336No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG106330.11518660598489369No Hit
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC106200.11504577781995401No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA104660.11337750571220703No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA101440.1098893003960088No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC98180.1063577633367522No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG98140.10631443159369386No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA97860.10601110939228531No Hit
GCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGG97310.10541529792523281No Hit
ACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCC97250.10535030031064527No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC97140.10523113801723476No Hit
GCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCC97120.1052094721457056No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC96970.10504697810923673No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA95020.10293455563514153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)