FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333049
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN404720.9340305175408817No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT85110.19642058051962946No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT65680.15157917669520932No Hit
CTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTC57290.1322163677355137No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA56990.1315240146141897No Hit
GGCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATT54670.12616981714261713No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT50560.11668457938047781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)