FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5163727
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1128512.185456357394572No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA158970.3078590328264837No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT88160.17072939758434172No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG64760.125413291601202No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC60040.11627260697554306No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT52370.10141899445884725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)