FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4716864
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1318332.7949290036770194No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT237260.5030036905876447No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG170860.36223219494986497No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA146130.30980329303537263No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC132990.28194580127813734No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA125850.26680862539178574No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT117840.24982700370415598No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT96360.20428827288639229No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA96020.20356745498704226No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG93140.19746170336901805No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG87870.18628902592909186No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA87760.18605582013812566No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT87330.18514419750071234No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGG84730.1796320606233294No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG83880.1778300158749542No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT82240.17435312953691268No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT78820.16710254949050893No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA78320.1660425231679353No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG77730.1647916921072984No Hit
GGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTGGGACTGTCTAC75290.15961876365313904No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG74000.15688389574089903No Hit
GTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACC71300.15115975359900136No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG70930.15037533412029686No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA68910.14609282777709937No Hit
CCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAA68750.1457536193538758No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT66350.1406654930055223No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC65990.1399022740532693No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT63660.13496255139007612No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG62350.1321852824249332No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT60340.12792397660818713No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG59790.12675794765335613No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA59130.12535871290755893No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG57000.1208430007733952No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT56420.11961337023920979No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT55040.11668769758890654No Hit
GTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCC54500.115542869160527No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG54300.11511885863149754No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA53400.11321081125086499No Hit
GGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGG53050.11246879282506345No Hit
GCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTA52920.11219318598119428No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG52490.11128156334378095No Hit
GTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACA52260.11079395123539708No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA52170.11060314649733383No Hit
GACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTAC49800.10557862172833475No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG49670.10530301488446561No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG49580.10511221014640236No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA49560.1050698090934994No Hit
GGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAG49380.1046881996173729No Hit
GTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGG48430.10267414960448298No Hit
ACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTG47920.10159292275545785No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA47340.10036329222127244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)