FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834978
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1332322.755586478366603No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT240580.49758240885480765No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG174220.36033255994132757No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA150490.3112527089058109No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC137330.2840343844377368No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA130780.2704872700558307No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT120650.24953577865297422No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA102730.21247252831346908No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT98790.2043235770669484No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG96150.198863366079432No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA93420.193217011535523No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG92860.1920587849624135No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG90120.18639174780112755No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT89710.18554376048867233No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGG89350.1847991862631019No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT84620.1750163082438017No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT81650.1688735708828458No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA81560.1686874273264532No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG80300.16608141753695674No Hit
GGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTGGGACTGTCTAC78090.16151055909664946No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG75270.15567806099634787No Hit
GTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACC73910.15286522503308184No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG73790.15261703362455836No Hit
CCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAA72940.1508590111475171No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA71530.14794276209736631No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC68020.14068316339805476No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT67820.14026951105051566No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT67190.13896650615576742No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG63440.1312105246394089No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG62900.1300936633010533No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA62440.1291422629017133No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG59430.12291679507124954No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT59400.12285474721911867No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT58830.12167583802863219No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG58330.1206417071597844No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT57350.11861481065684269No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA56570.11700156650144014No Hit
GTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCC56360.11656723153652407No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA55080.1139198565122737No Hit
GGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGG54780.113299377990965No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG54420.11255480376539458No Hit
GCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTA53930.11154135551392375No Hit
GTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACA53750.11116906840113853No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA52050.10765302344705602No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG50850.1051711093618213No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG50770.10500564842280564No Hit
GGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAG50770.10500564842280564No Hit
GTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGG50730.10492291795329782No Hit
GACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTAC50170.10376469138018829No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA49960.10333035641527222No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT49930.10326830856314134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)