FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834978
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1318572.7271478794732884No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA191460.3959893922991997No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG149580.30937059072450795No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG135560.2803735611620156No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA87340.18064198017033378No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT84870.17553337367822563No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC72880.15073491544325537No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC70120.14502651304721553No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG66620.13778759696528092No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA66350.1372291662961031No Hit
CAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC65660.13580206569709313No Hit
GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCAC61530.1272601447204103No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT57650.11923528917815136No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT57380.11867685850897357No Hit
GACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTC56630.11712566220570186No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG56570.11700156650144014No Hit
CCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT56050.11592607039783841No Hit
CCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAG55630.11505740046800626No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG53560.11077609867097638No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG53000.10961787209786684No Hit
GAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT52650.10889398048967339No Hit
CACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCA52550.10868715431590383No Hit
GAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCT52200.10796326270771037No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTAT50570.10459199607526654No Hit
CAACAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATAC49530.10244100386806311No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA48420.10014523333922098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)