FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4889186
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1363712.7892373086235622No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA189390.38736509513035505No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG148750.3042428739671594No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG136250.2786762459026922No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA89160.18236164465823143No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT84390.17260541938883078No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC73300.14992270697003549No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC68430.1399619486761191No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG67760.13859157741186365No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA65980.1349508895754835No Hit
CAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC63980.13086022908516878No Hit
GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCAC58670.11999952548338312No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG57630.11787238202841946No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT57570.11774966221371001No Hit
CCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAG57500.11760648909654899No Hit
GACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTC57290.11717696974506596No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT57180.11695198341809863No Hit
CCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT54370.1112046054292064No Hit
CACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCA53660.10975242095514469No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG52160.10668442558740862No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTAT51530.10539586753295947No Hit
GAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCT51510.10535496092805632No Hit
GAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT51010.10433229580547763No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG50930.10416866938586504No Hit
CAACAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATAC49240.10071206127154908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)