FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4931742
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1445742.9314996607689534No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT244300.4953624905763522No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG174380.35358702868073794No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA152880.3099918852202731No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC137270.27833978338688437No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA134270.2722567401133311No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT124270.25197992920148704No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA102480.2077967582245787No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT98940.20061876716178587No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG96660.19599565427388535No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA94270.1911494964659546No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG91860.1862627850362002No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG91780.18610057054890544No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT91480.1854922662215501No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGG90570.1836470764285723No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG82050.1663712335316811No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT81580.16541822341882442No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA81440.1651343480660586No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT81070.16438410606232037No Hit
GGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTGGGACTGTCTAC80470.16316749740760972No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG77270.1566789179158196No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG76790.1557056309920511No Hit
GTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACC75880.15386044119907327No Hit
CCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAA74240.15053504420953084No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC71340.14465476904509603No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA71040.1440464647177407No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT70960.14388425023044596No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT65520.13285366509440275No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT63470.1286969188574747No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG63390.12853470437017994No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA62790.12731809571546931No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG62240.1262028711153179No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT59620.1208903466564147No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG58560.11874100469975923No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG58240.11809214675058022No Hit
GTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCC57570.11673360041948666No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA57310.11620640333577872No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT56580.11472619613921409No Hit
GCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTA56090.11373263240453374No Hit
GGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGG55730.11300266721170735No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA54950.11142107596058351No Hit
GTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACA54690.11089387887687555No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG54520.1105491730913742No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG53340.1081565094037766No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA53310.10809567897104107No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG52420.10629104279988695No Hit
GGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAG52260.10596661382529743No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT52180.10580439933800267No Hit
GACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTAC51290.10399976316684854No Hit
GTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGG51020.10345228927222876No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC50210.1018098675883694No Hit
ACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTG49620.10061353574457059No Hit
GTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGT49560.10049187487909952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)