FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930435
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1448992.938868477122201No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT248420.5038500659678101No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG177380.35976541623609276No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA150680.3056119794703713No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC138960.28184125741440663No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA134970.2737486651786303No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT126840.2572592479162589No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT101850.20657406496587014No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA101390.2056410844073596No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG96390.19549999138007093No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT94880.19243738128582974No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG93710.1900643655174442No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA93440.18951674649397063No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGG90800.1841622493755622No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG89640.18180951579323124No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT84680.17174955151016086No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA84310.1709991106261415No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT83360.16907230295095665No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG82760.16785537178768203No Hit
GGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTGGGACTGTCTAC81170.16463050420500422No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG78480.15917459615632293No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG76550.1552601342477895No Hit
GTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACC74760.15162962294402016No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA74310.15071692457156416No Hit
CCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAA72260.14655907643037583No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT69650.14126542587013113No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC69440.140839499962985No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT67410.13672221619390582No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG66890.1356675425190678No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG64650.13112433284284247No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA63130.1280414405625467No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT61880.12550616730572453No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT59770.12122662604820873No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG59020.11970546209411544No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG58370.1183871200005679No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA57350.11631833702300103No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT56630.11485801962707144No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG56260.11410757874305208No Hit
GCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTA55850.11327600911481442No Hit
GGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGG55730.11303262288215948No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA55570.11270810790528624No Hit
GTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCC55290.11214020669575808No Hit
GTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACA54250.11003085934608203No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA53050.10759699701953276No Hit
GACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTAC52930.10735361078687783No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG52380.10623809055387609No Hit
GGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAG51940.10534567436747468No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG51910.10528482780931094No Hit
GTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGG51340.10412874320420004No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA50310.10203967804057858No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA50300.10201939585452399No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT49980.10137036590077751No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC49950.10130951934261379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)