FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878880
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1410752.891544780769357No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA189490.3883883186305054No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG147720.3027744072410061No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG135290.27729724854884724No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA88400.18118912537303644No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT84060.17229364116354573No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC74210.15210458137933294No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC69380.1422047683074804No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG67150.13763404715836422No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA65750.13476453612304462No Hit
CAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC64570.13234594825041812No Hit
GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCAC60710.12443429639589414No Hit
CCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAG58200.11928967303971402No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG57140.11711704325582922No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT57020.11687108516708752No Hit
GACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTC56920.11666612009313612No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT56250.11529285409766175No Hit
CCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT55960.11469845538320271No Hit
GAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCT53080.10879546125340243No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG52270.1071352441543961No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG52010.10660233496212244No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTAT51870.1063153838585905No Hit
GAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT51540.10563899911455088No Hit
CACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCA50870.10426573311907651No Hit
CAACAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATAC49150.10074033384711244No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA48850.10012543862525825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)