FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4961548
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1429692.8815401967289245No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT250430.5047416653028449No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG177580.3579124902147475No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA153250.3088753751853252No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC139760.28168628016901176No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA134580.2712459901627476No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT129410.26082585515649553No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA101960.20550038012330024No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT99650.20084457512050674No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG97310.19612830511767698No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA95080.19163374011498024No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT94400.19026320011415793No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG93850.18915467511349282No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGG91540.18449887011069932No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG90720.1828461601097077No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT86250.17383687510430212No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA84750.17081362510248818No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG83380.16805239010083142No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT82070.16541208509924724No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG78610.15843845509506307No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG78570.1583578350950147No Hit
GGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTGGGACTGTCTAC78440.1580958200948575No Hit
GTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGCAACC75340.15184777009110867No Hit
CCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAA72830.14678886508807332No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA72740.1466074700879645No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC71110.14332220508599333No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT70220.14152841008491707No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT67170.13538113508122868No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG65410.1318338550791003No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG63750.1284881250770929No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA63500.12798425007679054No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT62750.12647262507588355No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT60310.12155480507293288No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG59770.12046643507227987No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG59720.1203656600722194No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA58040.11697962007018778No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT57540.11597187006958311No Hit
GCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTA57460.11581063006948639No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA56970.11482303506889383No Hit
GTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCC56810.11450055506870033No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG55460.11177963006706777No Hit
GGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGG55440.1117393200670436No Hit
GTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACA54590.1100261450660157No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG54010.10885715506531429No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA53860.10855483006513289No Hit
GACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTAC53720.1082726600649636No Hit
GGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAG53550.10793002506475802No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG52900.10661995006397197No Hit
GTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGG52440.1056928200634157No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT51900.10460445006276267No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC51480.10375794006225475No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA50970.10273003506163803No Hit
GTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGT50580.1019439900611664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)