FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219736
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCA64510.12358862593817005No Hit
CGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCT62360.11946964367546557No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT62350.11945048561842973Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)