FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5416737
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG127460.23530771385060784No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT125720.23209544786833847No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT88620.16360402951075528No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT63480.11719232445658706No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT63390.11702617276784899TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGG60220.11117394106451911No Hit
GCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGGG58280.10759244910727621No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC54180.10002331662031959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)