FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361498
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG127180.23720982456768613No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT124860.23288267569996296No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT88190.16448761148470073No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT63830.11905254837360753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT63450.11834379123148045TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGG60660.11314002168796855No Hit
GCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGGG59390.11077128071296492No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC54860.10232214951866064No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG53630.10002801455861775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)