FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5356904
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCA67240.12552026319680173No Hit
CGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCT66850.12479223073626108No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT65020.12137607842141655Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)