FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6363516
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT280230.4403697578508485No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT204860.32192894619892526No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA98640.15500864616353602No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC97620.15340575870320747No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG94290.14817280258272314No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT93810.14741850260139205No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC90010.14144696108252106No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG85580.134485400838153No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG82490.12962959470833418No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA78990.1241294906777951No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT77260.12141086782841434No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT75900.11927368454797631No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG75130.1180636616612577No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA74870.11765508250470337No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT74690.11737222001170422TruSeq Adapter, Index 19 (97% over 38bp)
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG74660.11732507626287102No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA70040.11006493894255942No Hit
GGCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATT70000.11000208061078184No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT69490.10920063688061757No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG68180.10714202651490151No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG66650.1047376953244087No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG64990.10212907455563874No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT64060.10066761834180978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)