FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6552827
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA156900.2394386422837044No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG134650.20548383163480435No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG123170.18796467539887746No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT83050.1267391921074675Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG79710.12164215536286857No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG70970.10830440052819951No Hit
CAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC68500.10453503503144522No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG67230.10259694022137315No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG65760.10035363363018739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)