FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6516618
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA154480.23705547877748856No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG132180.20283527437084695No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG118140.18129035643949054No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT80670.12379120580644745Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG79590.12213390442711236No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG70130.10761717197478814No Hit
CAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC67220.10315166548046854No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA65390.10034346036548406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)