FastQCFastQC Report
Fri 10 May 2024
EGAF00008016752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6504289
Total Bases656.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA149640.23006357804826938No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG131370.20197442026330625No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG118520.1822182255431762No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT78510.12070496867528488Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG76800.11807593420280063No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG68500.10531512360536255No Hit
CAACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC65830.10121013995534331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)