FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008016754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008016754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6470409
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA150960.23330828082119692No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG128460.1985345903172427No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG115710.17882949903166862No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT78400.12116699268933387Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG75500.11668505035771308No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG67070.10365650764889824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)