FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215017
Sequences flagged as poor quality0
Sequence length0-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT152820.29303835442914183No Hit
137100.26289463677683117No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG121800.23355628562668154No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG115310.22111145562900372No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC105300.20191688732750057No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT98500.18887762014965628No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGTAACTCC96700.18542604942610924No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT91060.17461112782566193No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA87620.16801479266510538No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT75070.14394967456481925No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG73940.14178285516614808No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG72570.1391558263376706No Hit
CCCCGAGGTAGCTTTTATCCGTTGAGCGATGGCCCTCCCACGAGGTACCA68150.13068030267207184No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG67380.12920379741811003No Hit
CCTCGCTTCAACCTGGCCATGGATAGGTCACCCGGTTTCGGGTCTACGGC66290.12711367959107325No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA64910.12446747536968719No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT64100.12291426854409103No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGG62930.12067074757378547No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT62300.11946269782054401No Hit
CCGGAGTAGTAAGTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGAT61680.11827382346021116No Hit
GGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTT60560.11612617945444856No Hit
ATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGA59070.11326904591106798No Hit
GGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT58220.11163913751383743No Hit
GGGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAA58000.11121727886984838No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG57140.10956819507970923No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA56110.10759312961012399No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCTACAACGT53960.10347042013477616No Hit
GTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTA52780.10120772377156201No Hit
CCTCGATCTATTAGTATCGGTCAGCTGAACATGTTACCATGCTTACACCC52570.10080504052048152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)