FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4783368
Sequences flagged as poor quality0
Sequence length0-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT132160.2762906805414093No Hit
129990.2717541280537061No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG106300.2222283545819598No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG105820.22122487753398862No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC92320.19300208555979803No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT85800.17937152232485565No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGTAACTCC80720.16875139023382688No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT78320.16373400499397076No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA78300.1636921934503053No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT66330.138667984566523No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG63050.13181089140538632No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG62630.1309328489884115No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA58110.12148344012001586No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGG56350.11780402427745472No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG55990.1170514164914763No Hit
CCTCGCTTCAACCTGGCCATGGATAGGTCACCCGGTTTCGGGTCTACGGC54970.11491902776453745No Hit
CCCCGAGGTAGCTTTTATCCGTTGAGCGATGGCCCTCCCACGAGGTACCA54880.11473087581804285No Hit
GGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTT54770.11450091232788279No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT53230.11128142346564177No Hit
CCGGAGTAGTAAGTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGAT53190.11119780037831084No Hit
ATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGA53030.1108633080289871No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT52420.10958805594719036No Hit
GGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT51720.10812465191889899No Hit
GGGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAA51650.10797831151606985No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG51410.10747657299208424No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA50170.10488425728482524No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCTACAACGT48470.10133027607326052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)