FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319970
Sequences flagged as poor quality0
Sequence length0-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT154980.29131743224115925No Hit
145130.2728022902384788No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG118780.22327193574399853No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG115250.21663655998060138No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC104430.1962980994253727No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT98310.18479427515568697No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGTAACTCC97480.18323411598185704No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT94250.17716265317285623No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA86590.1627640757372692No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT76610.1440045714543503No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG73930.13896694906174284No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG71780.1349255728885689No Hit
CCTCGCTTCAACCTGGCCATGGATAGGTCACCCGGTTTCGGGTCTACGGC68960.12962479111724315No Hit
CCCCGAGGTAGCTTTTATCCGTTGAGCGATGGCCCTCCCACGAGGTACCA68380.12853455940541017No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG66190.12441799483831675No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA66000.12406084996719907No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT63720.11977511151378673No Hit
GTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGG62660.1177826190749196No Hit
CCGGAGTAGTAAGTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGAT62300.11710592352964395No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT61860.1162788511965293No Hit
GGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTT60520.11376004000022556No Hit
ATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGA59880.11255702569751333No Hit
GGGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAA59270.11141040269024073No Hit
GGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT57940.10891038859241688No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG56720.10661714257787168No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCTACAACGT56030.10532014278276006No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA55790.10486901241924297No Hit
GTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTA54010.10152312888982457No Hit
GTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAAT53610.10077124495062942No Hit
CCTCGATCTATTAGTATCGGTCAGCTGAACATGTTACCATGCTTACACCC53580.10071485365518978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)