FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319970
Sequences flagged as poor quality0
Sequence length0-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
145680.2738361306548721No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA142180.26725714618691454No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG102770.19317778107771283No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG94450.17753859514245382No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC74560.14015116626597518No Hit
CCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGTC72780.1368052827365568No Hit
CAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGAT65400.12293302405840634No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTAT65110.12238790820248986No Hit
GAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT60070.11291417056863104No Hit
ATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTA59760.11233146051575478No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA59060.11101566362216328No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT57090.10731263522162719No Hit
AATTAATTAACCACTCATTCATCGACCTCCCCACCCCATCCAACATCTCC56870.10689909905506986No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG54170.10182388246550263No Hit
GTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTG53820.10116598401870688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)