FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780121
Sequences flagged as poor quality0
Sequence length0-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA128500.2688216469834132No Hit
123220.2577759014886861No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG92690.19390722536103167No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG83360.17438889099250834No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC65750.1375488193708904No Hit
CCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGTC65290.13658650063460737No Hit
CAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGAT57120.11949488307931955No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTAT56740.11869992412325964No Hit
GAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT54360.11372097066162133No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA53190.11127333387585797No Hit
ATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTA51030.10675461980983326No Hit
AATTAATTAACCACTCATTCATCGACCTCCCCACCCCATCCAACATCTCC50910.10650358013949855No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT50350.1053320616779366No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG48330.1011062272273024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)