FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8440660
Sequences flagged as poor quality0
Sequence length0-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATGCAA285680.3384569453099639No Hit
GGCGGATGCCTTGGCTCTCGGAGGCGATGAAGGACGTGATAAGCTGCGAT206300.24441216682107797No Hit
GTGAAATAGAACCTGAAACCGTGTGCCTACAACCGCTCAGAGCCCCTTAT149250.17682266552615555No Hit
GCCACATACTTTGAGCTCGGCAGTCAGACTACGAATGATAAGATCCGTGG126930.15037923574696765No Hit
GTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAA110560.13098501775927476No Hit
GTCGTACCGTAAGGAGCGGTGGACTGGTTACGAGTGAGAATGTTGGCATC102260.12115166349550865No Hit
GAGCGTCGTAACCGGGCTGAAGTCGTACCGTAAGGAGCGGTGGACTGGTT100890.11952856767124846No Hit
CATACTTTGAGCTCGGCAGTCAGACTACGAATGATAAGATCCGTGGTCAA98510.11670888295465046No Hit
CCAGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTGTAG95940.11366409735731565No Hit
GGTGAATGCCTTGGCATCAGGAGCCGATGAAGGACGTGATAAGCTGCGAT95430.11305987920375894No Hit
93400.11065485400430773No Hit
GACCGAACCACGTTGCTGTTGAAAAAGCATGGGATGAGCTGTGGATAGCG90440.10714801923072366No Hit
CACATACTTTGAGCTCGGCAGTCAGACTACGAATGATAAGATCCGTGGTC88640.10501548457111175No Hit
AATGACGGTACCCAAGGAGGAAGCCACGGCTAACTACGTGCCAGCAGCCG84720.10037129797906799No Hit
GTCGGCTTAAGTGCCATAACGAGCGCAACCCTTATCTTTAGTTACTAACA84630.1002646712460874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)