FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4404325
Sequences flagged as poor quality0
Sequence length0-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
149070.33846276103602707No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA126000.2860824303383606No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG80830.1835241495575372No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG80100.18186668785795781No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT61460.13954465213171147No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC55960.12705692699789412No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG55930.12698881213352783No Hit
GCTTCACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCA52970.12026814551605525No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG52850.11999568605859015No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA50100.11375182349168146No Hit
CAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGAT45700.10376164338462761No Hit
GTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTATTCTGCCT44930.10201336186589319No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT44700.10149114790575173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)