FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973915
Sequences flagged as poor quality0
Sequence length0-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
137650.34638385571910824No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT123070.30969459588340464No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG92420.2325666251039592No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC81490.20506226227787963No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG80130.20163994448799233No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT72080.18138284286402703No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT67300.16935440239662902No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG60570.15241896215696613No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT59310.14924828538104112No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG57710.1452220291576443No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG56590.1424036498012665No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA51600.12984676320454766No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA51450.1294693016836042No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT51140.12868921454032106No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT50990.1283117530193776No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA50260.12647477361745282No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT49960.12571985057556592No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA49910.12559403006858477No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA49630.12488943522949031No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG48100.12103932771586709No Hit
GCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAAT47460.11942882522650838No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA46800.11776799453435717No Hit
GGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGA45050.1133642767900169No Hit
GGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTT44480.11192992301043178No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA44060.11087303075179011No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG43900.11047040512945042No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG43830.11029425641967683No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA43340.10906121545126153No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT42740.10755136936748773No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG42590.10717390784654429No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA42470.10687193862978953No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA42070.10586537457394031No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT40670.10234240037846809No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG40630.10224174397288316No Hit
CTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAA40390.10163780553937363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)